Materials

Uric acid, creatinine and creatinine-d₃ were purchased from Wako Pure Chemical Industries (Osaka, Japan). Louis, MO). Other reagents and solvents were of analytical grade.

Animals

Seven-week-old men Wistar rats was basically ordered off Sankyo Labo Solution (Tokyo, Japan). The animal knowledge was authorized by the Panel away from Kanazawa College with the Worry and employ from Research Dogs and had been performed relative to the direction (AP-143148). Mice was in fact located two to four per cage which have 100 % free access to help you h2o and you may a commercial creature chow from the acclimatization and you will fresh episodes; these were handled for the an effective twelve-hr ebony/white cycle within the an atmosphere-controlled place (temperature, twenty four.0 ± 10°C; moisture, 55 ± 5%). Leggi il resto di questo articolo »